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Additionally, the protective effects of estrogens in PD are also related to the activation of the mitogen-activated protein kinase (MAPK) signaling pathway and the consequent upregulation of Bcl-2, as demonstrated in dopaminergic cells in mesencephalic slice cultures (Wang et al., 2011)

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Each biopsy was fixed in 10% formalin, routinely processed, embedded in a paraffin block, sectioned by the ordinary microtome into 5 m thick sections and stained with hematoxylin and eosin (H and E) and Masson fontana (MF) for detection of melanin