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The cDNA for SLC3A2 coding region was amplified from ZR-75-1 breast cancer cell line using standard RT-PCR and ligated into BamH I- EcoR I linearized pBabe-puro retroviral vector, generating the pBabe-puro-SLC3A2

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GHK-Cu is photolabile as a copper(II) peptide complex, so keep both lyophilised vials and reconstituted solution protected from UV and fluorescent light, using amber or foil-wrapped vessels for aliquots