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Improvement of liver enzymes was explained by the direct action of GLP-1RAs on GLP-1 receptors located on the hepatocyte that enhances weight loss, insulin sensitivity, hepatic fat oxidation, reduces denovo lipogenesis, hepatic steatosis, and alleviates oxidative stress [27,28,29,30,31,32,33,34]
Thus, mutations in these critical areas are possible without completely disrupting the function of G6PD
The cells were first collected by centrifugation at 12,000 g for 5 min, resuspended in RNA later Solution (Ambion), and harvested from the RNA later suspension by centrifugation after overnight storage at 4C