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In order to simply compare the NAD(P)H oxidation activity of the wild type and mutants of cFSP1 or hFSP1, we chose to compare the initial activity that was derived from the decrease in the absorbance at 340 nm within five minutes of the reaction

(2) DHODH: DHODH is a mitochondrial flavoprotein localized to the inner mitochondrial membrane, where it catalyzes the fourth and rate-limiting step of de novo pyrimidine biosynthesisthe oxidation of dihydroorotate to orotate 42,43