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The uptake of glucose into the brain is also reduced in aged healthy individuals as well as aged rodents indicating there may be a change in expression or function of the GLUT1 transporter with age [91,92,93,94]

123 TMAO may influence the crosstalk of different cells in the NVU and participate in the occurrence and development of neurological disorders

These observations were further confirmed by Hoechst 33342 staining, which revealed extensive detachment of cells from the cell culture substratum and increases in apoptotic bodies of HepG2 cells incubated with H 2 O 2 and TNF for 16 h (Fig