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Slides were also counterstained with mouse anti-insulin primary antibody (1:1000, 4 C overnight) and goat anti-mouse secondary antibody conjugated with Alex Fluor 594 (1:400, 1 h at room temperature)

In that study, the glucose-sensing transcription factor carbohydrate response element binding protein (ChREBP) has been shown to initiate a regulatory program driving an early transcriptional activation of metabolic genes, which is followed by a second wave of transcriptional regulation wherein cell-cycle genes are activated and -cell identity genes are repressed

Blood pressure monitoring is advisable

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