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Statistical analysis Statistical analyses were performed using the one-sample t -test for quantification of the band intensity of western blots, the two-sample t -test for comparing the means of two groups, and the one-way ANOVA with Dunnetts multiple comparison for comparing means among multiple groups

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Common reasons for an imbalanced ratio between phosphatidylcholine and cholesterol include: Fasting Overeating High-fat diet Ketogenic diet Vegan diets Protein deficiency Low dietary choline intake Low dietary glycine intake Methylation disregulation Dirty MTHFR Dirty PEMT Dehydration Low stomach acid Ongoing intake of proton pump inhibitors (PPIs) Bacterial overgrowth of specific bacteria such as certain Clostridia species What Happens When You Dont Have Healthy Bile Composition or Bile Flow

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