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Concurrently, short-chain fatty acid standards (Sigma-Aldrich), GLP-1 ELISA kit (ab226922, Abcam), Oil Red O Staining Kit (G1262, Solarbio), RIPA Lysis Buffer (P0013B, Beyotime), TRIzol Reagent (15596026, Invitrogen), and anti-GLP-1 antibody (ab23468, Abcam) were utilized according to manufacturer specifications

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