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Genome editing with CRISPR-Cas nucleases, base editors, transposases and prime editors

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Generation of NEDD8-conjugated CUL1-RBX1 CUL1 neddylation was performed by incubating 8 M CUL1-RBX1, 500 nM APPBP1-UBA3, 1 M UBC12, 10 M NEDD8, and neddylation buffer (50 mM Tris pH 7.6, 100 mM NaCl, 2.5 mM MgCl 2 , 150 uM ATP) at room temperature for 10 min

JMIR Hum Factors 10:e45143 Jaiswal V, Gurudiwan P (2023) Identifying thyroid dysfunction using standard laboratory testingsa systematic review