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Longevity context: Preserving lean muscle mass, improving sleep architecture, and supporting fat metabolism are all documented contributors to healthspan and functional longevity

In this context, lifestyle interventions include weight loss through a healthy diet, decreased consumption of ultra-processed and fructose-laden foods such as sugar-sweetened beverages, and increasing physical activity [25]

From 4-week old, mice were fed with a high-fat diet (D12492, Research Diets, USA) for 5 months, switched to above MCR or CTR diets for 4 months, and then switched back to HFD for additional 2 months

Swelling of the optic nerve head: a staging scheme