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The absorbance (OD 600 ) was measured hourly using a FLUOstar Omega plate reader (BMG Labtech), with results presented as mean values

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Quantitative analyses of fresh eggs from 50-week-old Cobb 500 breeder hens indicate that the yolk accounts for over 94% of total Mn and P, while contributing less than 20% of total Na and approximately one-third of total K, highlighting the yolks primary function as a storage reservoir for trace minerals essential to embryonic development (146)

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