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The choice of manufacturing approach depends on factors such as peptide length, complexity, and required production volumes

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For lentiviral particles production, the full-length cDNA-encoding mito GCL, or its mutant inactive E103A form, was first digested with Eco 47III /Sma I to generate blunt ends, and then subcloned in the Pme I site of pWPI lentiviral vector (Didier Trono laboratory, Lausanne, Switzerland)

J., Doll, M