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For immunoblotting, 20 g per specimen was separated on 420% gradient precast gels (Mini-PROTEAN TGX, Bio-Rad) and transferred to polyvinylidene difluoride membranes (Immobilon -P, Merck Millipore)

Here's the honest picture: Strong: Consistent positive results in animal studies Multiple mechanisms that make biological sense Good safety profile in research settings Decades of study without major red flags Weak: Limited human clinical trials Not FDA approved for any indication Long-term effects unknown Quality control issues with commercial products The peptide is stuck in an odd place

Two safety topics deserve emphasis: TMAO and cardiovascular signals

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