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The samples from 3 DIV neuronal cell cultures after fixation, permeabilization and blocking were incubated overnight with neuronal (antiMAP-2, 1:250) and astrocyte (antiGFAP, 1:500 ) -specific antibodies followed by 60 min labeling with secondary antibodies (antimouse Alexa Fluor488 and antirabbit Alexa Fluor568, dilution 1:500)

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Common carotid arteries occlusion surgery in adult rats as a model of chronic cerebral hypoperfusion

However, tirzepatide already holds FDA approval specifically for type 2 diabetes (Mounjaro), and its glycemic efficacy data is incredibly robust