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While GHK-Cu is generally well-tolerated in topical research, certain contraindications or precautions should be considered, especially for systemic research or in specific populations: Systemic Research: For systemic applications, GHK-Cu is generally not recommended for subjects who are pregnant, breastfeeding, have active cancer, or suffer from Wilsons disease (a condition involving copper accumulation)

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The topological properties of the drug-disease network were analyzed using Cytoscape software, as demonstrated in Figure 5D

In a rat ischemic wound model, topical tripeptide-copper complex reduced wound area more than vehicle and was associated with lower TNF-alpha and MMP-2 and MMP-9 concentrations at measured time points 16