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(3) from arcade 2 to the next arcade (arcade 3) to the last arcade inside the ligations (or the previous ligation) (2-3, Figures 2, 5-8, 21 and 22)

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Afterwards, the sample homogenate was heated in a boiling water bath for 10 min, cooled in an ice water bath, and transferred to a 50-mL amber centrifuge tube

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