Viable hepatocytes were resuspended in Hepatocyte Medium (Williams E, 5% FBS, 10 nM insulin, 100 nM dexamethasone, 100 nM triiodothyronine, and 100 g/mL penicillin-streptomycin), or, for Mpc fl/fl and liver-specific knockout ( Mpc2 LKO ) hepatocytes, in high glucose DMEM, 10% FBS, penicillin-streptomycin, and 0.5 g/ml amphotericin B, and plated on collagen-coated tissue culture plates
The analysis utilizes a gradient method with a simple mobile phase consisting of water, acetonitrile (MeCN), and TFA
In adults (offlabel): may support favorable changes in body composition, energy, recovery, and metabolic markers (evidence limited
These drugs could also be used in kidney and liver transplant recipients with new-onset diabetes after transplantation (NODAT) due to all these benefits