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Commercial test kits have simplified the assays [33], but they still require a biochemistry laboratory and a skilled technician. Beutlers fluorescent spot test is a popular screening test in which haemolysate is incubated with G6PD reaction substrates is placed on filter paper and illuminated with UV light (450 nm) [34]

The mixture containing: 1 FastStart Universal Probe Master (Rox) mix (Roche, Mannheim, Germany), 900 nM TaqMan forward and reverse primers and 250 nM of hydrolysis probe labeled with the fluorescent reporter dye FAM at the 5-end and a quenching dye at the 3-end and RNAse free water

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In contrast, the cyanobacterial lineage A