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5-Amino-1MQ powder should be stored at room temperature (15-25C) in an airtight container, protected from light and moisture

The desalted sample was evaporated to dryness in a speedvac and then resuspended in 10 L Buffer A and analyzed by mass-spectrometry using the following LC-MS gradient: 5% buffer B in buffer A from 0-15 min, 515% buffer B from 1517.5 min, 1535% buffer B from 17.592.5 min, 3595% buffer B from 92.595 min, 95% buffer B from 95105 min, 955% buffer B from 105107 min, and 5% buffer B from 107125 min

Notoriously, non-motor symptoms are developed by many years before starting motor symptoms

34 It is crucial to promote proper hydration by frequent intake of water and other fluids, and to avoid physical activity and extreme weather that result in excessive sweating