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This mismatch between ideal diagnostic conditions and real-world practice contributes to false-negative assessments (Goncalves et al., 2010)

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Samples were fixed in 10% buffered formalin overnight at 4C, dehydrated with increasing concentrations of ethanol, embedded in paraffin, cut into 5 m sections, and stained with hematoxylin and eosin (HE) or Massons Trichrome Stain Kit (Sigma-Aldrich)

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