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Trotta et al (141) demonstrated that diabetic mice with heightened ERS markers [phosphorylated (p)ERK, phosphorylated inositol requiring enzyme 1 and activating transcription factor 6], increased NLRP3 inflammasome activity (NLRP3, apoptosis associated speck-like protein containing a CARD and caspase-1) and increased levels of pro-inflammatory cytokines (IL-1 and IL-18) experienced significant reductions in these parameters following intraperitoneal injections of 50 and 100 mg/kg -HB

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For immunofluorescence microscopy analyses, after antigen retrieval using citrate buffer, 5-m formalin-fixed paraffin embedded (FFPE) pancreatic sections were incubated with the indicated antibodies

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