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Then, 1 mL of the overnight culture was transferred into 50 mL of LB or M9 medium in 250 mL flasks for further cultivation (kanamycin or ampicillin was added when needed)

Modern approaches include debulking surgery, platinum/taxane chemotherapy, angiogenesis inhibitors (e.g., bevacizumab), drugs targeting poly(ADP-ribose) polymerase (i.e., PARP inhibitors), and drugs targeting the dysfunctional immune system (i.e., immune checkpoint inhibitors) depending on the histological type and tumor stage [2]

Most cases are mild and resolve within 12 weeks as your body adapts to lower appetite and reduced calorie intake

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