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(b) oxidative stress including increased activity of pro-oxidative enzymes including myeloperoxidase (MPO) and oxidative damage as indicated by elevated production of malondialdehyde (MDA), protein carbonyls (PCs) and advanced protein oxidation products (AOPP)

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Cell transfection The shVRK2-expressing and VRK2-overexpressing plasmids were purchased from Genechem (Shanghai, China) and then used for constitutive expression of shRNA or cDNAs