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Mechanism: Antioxidant and cellular protective mechanisms may shield retinal pigment epithelium

Enzymes such as urease and catalase can lose up to 50% of their potency after shaking, fibrinogen clotting activity is decreased after shear stress, and recombinant IL-2 and recombinant interferon activity is substantially affected by aggregation from shaking and shearing (2)

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Pechkovsky DV, Hackett TL, An SS, Shaheen F, Murray L, Knight D: Human lung parenchyma but not proximal bronchi produces fibroblasts with enhanced TGF-beta signaling and alpha-SMA expression