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Antioxidant enzyme activity assay For the enzyme assays, 0.3 g of leaf sample was ground in 3 mL of ice-cold 25 mM HEPES buffer (pH 7.8) containing 0.2 mM EDTA, 2 mM AsA, and 2% PVP

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In a large, rigorous review of over 50 studies involving more than one million patients, metformin use in patients with type 2 diabetes was associated with a 35% reduction in risk of death from cancer, and a 27% reduction in risk of any cancer (Franciosi 2013)

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