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ARRIVE guidelines statement : The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines

The supernatant was transferred to vials for MS analysis

Fourteen genes whose expression is significantly correlated with regional emphysema severity or transcription factors that are highly connected to these genes in the relevance network were selected for quantitative RT-PCR validation in a subset of tissue cores from subjects with severe emphysema (see Additional file 1 for methods)

Skip aspiration for subcutaneous shots it isnt needed [7]