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Representative field images of four caudal nerves from each tail were taken using light microscopy (magnification 500) with a CCD camera using ISSA 3.1 software (VamStec, Zagreb) according to a previous study [41]
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The DIA method included a survey scan (380980 m/z, resolution 240,000, AGC 500%, 5 ms injection) and MS/MS scans (1502000 m/z, 2 m/z isolation window, AGC 500%, 3 ms injection, normalized collision energy 25, cycle time 0.6 s)
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