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The treated fibroblasts were then trypsinized and seeded at 2 10 5 cells/500 l of 1% FBS DMEM, penicillin, streptavidin in duplicate on the collagen gels and cultured for an additional 24 h at 37C in 5% CO 2

Their approach involves the use of a novel lithium orotate derivative that has been chemically modified to enhance its ability to stimulate glutathione production

The PVDF membrane was subsequently washed (5 min 5) with tris-buffered saline with tween (TBST) buffer and then incubated with the appropriate secondary antibody (Peroxidase-conjugated goat anti-rabbit IgG (H + L), at a dilution of 1:5000) in a 5% bovine serum albumin solution for 1 h at 25 C

Nutrition 16 (10), 9961005