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Whole-cell Patch-clamp Recording Whole-cell membrane currents were recorded from rhodamine-labeled GCs using a patch amplifier (EPC9/2, HEKA Elektronik, Lambrecht, Germany) with Pulsefit 8.80 software (HEKA Elektronik)

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The volume of a saline flush can be determined by evaluating the blood flow rate and duration of saline flush.Haemoglobin ChangesWhen evaluating haemoglobin changes in a patient on CRRT, consider the Hb in relation to the start and stop times of a CRRT treatment