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400 MBT cases, the real story wasn't treatment time

After derivatization with 10 l of 5 mM 7-diethylamino-3-(4-maleimidophenyl)-4-methylcoumarin (Sigma-Aldrich) at room temperature for 10 min, the maleimide ring was cleaved by addition of 30 l of 50 mM ammonium formate (Sigma-Aldrich) pH 4.2 at room temperature for another 30 min

Taken together, these findings suggest that targeting RET-linked ROS occurring at the mitochondrial respiratory-chain complex 1 using metformin or metformin-like molecules might be therapeutically relevant in the context of both cardiometabolic and inflammatory diseases

These fat-soluble antioxidants have been studied for decades