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Lu et al., 2018)

the lysate was clarified by centrifugation at 15,000 nx g for 15 min, protein concentrations were determined using the BCA total protein assay (Pierce), and then appropriate volumes of the lysates were diluted with 2x reducing Laemmli buffer and heated for ten min at 95 C

Urey explains that, in reviewing the HELIOS B data, the goal was to better characterize under-recognized symptoms that may affect patients quality of life

Basically, when new cells are being created, growth hormone is part of that process