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See the 2025 BPC-157 literature and patent review by Jzwiak and colleagues in Pharmaceuticals (Basel) for the underlying chemistry

In the next day, 9 l of the drug dilutions (BSO, Auranofin, BSO+Auranofin) were added to the wells so that every drug/combination was tested at 5 (fixed concentration ratio, for combinations) concentrations (1:3 serial dilution) in 2 replicates