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about 2.4 mg total residual
Glutathione (GSH) is a tripeptide molecule made from three amino acids: Cysteine (rate-limiting amino acid-contains sulfur) Glycine Glutamate It exists in two forms: Reduced glutathione (GSH) : Active form, the antioxidant Oxidized glutathione (GSSG) : Inactive form, created when GSH neutralizes free radicals The GSH:GSSG ratio is a critical marker of oxidative stress
RT-qPCR was carried out using the 2 Hieff UNICON Universal Blue qPCR Master Mix (Yeasen, Shanghai, China) under the following amplification conditions: Uracil-DNA Glycosylase (UDG) activation at 50 C for 2 min, initial denaturation at 95 C for 2 min, then 40 cycles of 95 C for 15 s and 60 C for 30 s for denaturation and annealing/extension, respectively, concluding with a melting curve analysis
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