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In addition, in our assay we use an N-terminal peptide of the recombinant nAngptl4 protein to measure LPL activity in the presence and absence of nAngptl4, allowing us to calculate Angptl4-sensitive lipase activity, and therefore LPL-mediated hydrolysis

Additionally, although rare, there have been reports linking green tea extract supplementation to liver-related issues, particularly in individuals with sensitive metabolisms or those taking high doses for extended periods (Saha et al., 2024)

Calculation of Results The optical densities for the 15 measurements per sample are plotted against time (in minutes) from which the increase in OD (slopes) for each well can be calculated in OD/min