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8th edition, January 2020 OptiPrep Application Sheet C25 Hepatic non-parenchymal (stellate, Kupffer and endothelial) cells a short methodological survey This Application Sheet provides a review of the density gradient methods for purification of the three main types of non-parenchymal cells (stellate, Kupffer and sinusoidal endothelial)

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resultant supernatant fractions were centrifuged at 1,000 g for another 15 min, followed by centrifugation of the supernatant at 3,000 g for 15 min

doi: 10.1126/sciadv.abq5925 12 BestSAGubserPMSethumadhavanSKersbergenANegron AbrilYLGoldfordJet al