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(4) Notably, exposing wound models to the peptide has been linked with fewer infiltrating inflammatory cells and lower tissue levels of key inflammatory mediators such as leukotriene B4, thromboxane B2, and myeloperoxidase

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B), with the exception of using Qiagen HotStar MasterMix for the first PCR (amplicon PCR) and halving reagent volumes for the second PCR (index PCR)