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Quantification of H 2 S and total persulfide from mice heart exposed to Langendorff model of ischemia-reperfusion Snap frozen mouse heart obtained from Langendorff experiments was homogenised using Percellys tubes (approximately 10 mg tissue per 300 L of 50 mM HEPES (pH 7.4) buffer containing 10 mM IAM-TPP) two times for 30 s