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Description

Lentivirus production The following plasmids were used for lentivirus production: psPAX2 packaging plasmid (Addgene, #12260), pMD2-G VSV-G envelope plasmid (Addgene, #12259), Blast-Cas9 plasmid, pKLV2-U6gRNA5(gGFP)-PGKBFP2AGFP-W reporter plasmid (Addgene, #67980), and custom pooled CRISPR libraries

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However, side effects and metabolism differ for each person

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& Varmuza, K

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Rectovaginal examination, considered essential for detecting posterior disease, is often omitted in routine gynecologic care

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