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Glutathione -An amino acid and powerful antioxidant that Is essential to immune defense Zinc Sulfate -An essential mineral that may help combat infections and aid wound healing Ascorbic Acid -A strong antioxidant that contributes to immune defense by supporting various cellular functions of the immune system A powerful antioxidant comprised of amino acids that has many functions and is vital to mitochondrial function

The protein was then concentrated and loaded onto a Superdex 200 Increase 10/300 column (GE Healthcare) pre-equilibrated with buffer containing 20 mM HEPES, pH 7.5, 100 mM NaCl, 0.00075% (w/v) LMNG, 0.00025% (w/v) glyco-diosgenin (GDN, Anatrace), 0.0002% (w/v) CHS and 10 mM ligand