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The change of absorbance at 485 nm as a consequence of adrenochrome generation is indicative of superoxide formation ( 3.2 Hydrogen peroxide 3.2.1 Dichlorofluoresceine Frequently MitoSOX measurements are associated with staining with 5-(and -6)-carboxy-2,7-dichlorodihydrofluoresceine diacetate (DCFH-DA) for the evaluation of H 2 O 2 basal content