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pH Determination in Water and Culture Medium GHK, GHK-Cu, Cu(OAc) 2 and CuCl 2 samples were prepared and diluted in sterile water and culture medium (DMEM supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin) respectively to a final concentration of 580 M and 5800 M

The seven included studies are summarized in Table 1

Clinical studies show benefits at both ends of this range, with higher doses producing more dramatic results

Abbreviations ALP: alkaline phosphatase ALT: alanine aminotransferase AST: aspartate aminotransferase CKD: chronic kidney disease GGT: gamma glutamyl transpeptidase HGF: hepatocyte growth factor HPP: hypophosphatasia NSHA: nonspherocytic hemolytic anemia PLP: pyridoxal 5-phosphate TNSALP: tissue-nonspecific alkaline phosphatase Declarations Acknowledgement The support of the Herman Lopata Chair in Hepatitis Research is gratefully acknowledged