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Description

173 Combinatorial mutagenesis of the lid domain of Rhizopus oryzae lipase generated mutants that displayed a significant shift in substrate specificity toward short-chain substrates

Experiments to explore the substrate specificity at the terminal amino acid of all GS-like enzymes were carried out using 2.5 mM ATP and 1 mM -EC with the following amino acids at 100 mM: glycine, -alanine, D-alanine, GABA, AABA, diaminopropionic acid, D-serine)