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First, in the process of disrupting the IGF-1/IGFBP complex by acid/ethanol precipitation, there is the potential IGF-1 loss, which would directly affect the measurements accuracy

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A B12 shot is a quick intramuscular injection of vitamin B12 (cobalamin) that bypasses the stomach acid, liver metabolism, and intestinal absorption issues that limit oral B12 to roughly 1% bioavailability in most adults