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Agonist dissociation was monitored immediately after washing in 10 M exendin(9-39) by TR-FRET at 37 C, with 50 s delay and 300 s integration time, and binding quantified as 520 nm over 620 nm signal after nonspecific binding subtraction with excess (10 M) unlabeled agonist

Both groups had a balanced distribution of Fitzpatrick skin types 4 and 5, where type 4 was observed among 15 (75%), and type 5 was observed among the remaining 5 (25%) in each group