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For instance, the SKSC222 strain, engineered with a synthetic isozyme system, achieved 2.0 g/L of glutathione with a volumetric productivity of 29.85 mg/L/h under fed-batch fermentation conditions (Jeon et al., 2023)

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25,45 However, coupling DOTA either directly to the side chain of Lys or through a linker significantly diminished binding affinity (200- and 500-fold, respectively)

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