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Common Mistakes During Reconstitution Using tap water instead of bacteriostatic water Applying vigorous shaking instead of gentle swirling Failing to refrigerate the reconstituted solution Not labeling the vial with concentration or date Over-diluting or under-diluting the peptide solution Each of these errors can lead to inaccurate concentration, reduced peptide stability, or contamination

doi: 10.1002/ptr.8219 190 ZhuWWangMJinLYangBBaiBMutsinzeRNet al

10.1007/s00394-015-1042-7 263 ReczekC

The same principles apply when learning how to reconstitute peptides in general and how to mix peptides with bacteriostatic water