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The sediment was re-suspended in 20 mM HEPES, pH 7.5, 100 mM NaCl, 2 mM MgCl 2 , 50 mU/mL Apyrase (Sigma), and 100 mM ligand (CCK-8 or SR146131 or gastrin-17)

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Given the impact of post-translational modifications and other biochemical alterations (phosphorylation, methylation, alkylation), however, the predicted pI of a protein will certainly be different than the predicted pI

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Wie nimmt man Nac und liposomales l-glutathion

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