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Description

The procedure involves treating the skins middle layers by injecting a mixture of amino acids, vitamins and minerals

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Device preparation The fluidic channels were fabricated using a CO 2 laser cutter machine (100 W, CMA-1390 Yueming laser, Dongguan, China) on transparent sheets of polymethyl methacrylate (PMMA) and polyvinyl chloride (PVC, thickness = 100 m, GBC) and double-sided pressure sensitive adhesive (PSA, thickness, =80 m, Flexcon, Massachusetts, USA)

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This additional step of dissociation is required because effective transduction across all cells within the 3D structure is not yet feasible, making it difficult to preserve the organoids spatial organization and cell-cell interactions

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