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The function of ERK1/2 leads to an increase in cellular mitogenic messages, which is indirectly triggered by PKA through the increased intracellular calcium induced by PLC/PKC events, both of which, according to the description, have been stimulated by IGF activity [53, 65]

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Marshall et al., 2011)

Ni-NTA resin (2 mL), pre-equilibrated with 20 mM imidazole (all buffer contained 150 mM NaCl and 20 mM HEPES pH 7.5), was added into the supernatant for batch binding with stirring at 4 C for 1.5 h