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In a suitable tube (50 ml) layer 10 ml of each of the two gradient solutions and underlayer these with 10-15 ml of the sample-OptiPrep mixture (density approximately 1.17 g/ml), to form a three-step gradient (see Figure 1 and Notes 4 and 5)

Mater Adv 2:34413458 Huang RF, Jia B, Su DD, Li MC, Xu ZX, He C, Huang YS, Fan H, Chen HB, Cheng F (2023) Plant exosomes fused with engineered mesenchymal stem cell-derived nanovesicles for synergistic therapy of autoimmune skin disorders